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The best choice for purification of virus and vaccine——QuikSep Sulfate

2019年10月22日source:管理员
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In the field of biotechnology, affinity chromatography is often used to purify viruses and proteins. Affinity chromatography is a kind of adsorption chromatography, in which the target substances are selectively adsorbed on the ligands of column packing, and then the buffer is changed to achieve separation and recovery. Affinity chromatography is much more accurate than gel filtration or ion exchange chromatography in recovering target components.

QuikSep Sulfate is a specific affinity filler suitable for the isolation and purification of certain viruses, virus-like particles, and certain antigens and proteins (see table 2).


Features:

1. Quick and simple (one-step purification).

2. High load.

3. Easy to enlarge.


1:性能参数

介质

QuikSep Sulfate

基质

高刚性琼脂糖

粒径范围

45-165µm

平均粒径

90µm

溶菌酶载量

≥3mg/ml 介质

pH稳定性

5-12(长期) 5-12(短期)

操作压力

≤0.3MPa

流速

16mm× 300mm0.1MPa300cm/h

贮存溶液

20%乙醇

贮存温度

4-30

 

2QuikSep Sulfate适用性

Viruses

Viral/Microbial Agents

Rabies

Influenza  

Japanese   Enchephalitis

Feline   Leukemia

Feline   Herpes

Feline   Calicivirus

Respiratory   Syncytial Virus

Human   Herpes Simplex

Human   Measles

Human   Parainfluenza

Herpes   Simplex gA and gB Glycoprotein Subunits

Hepatitis   B Surface Antigen

Filamentous   Hemagglutinin from B. pertussis

Leucocytosis   Promoting Factor Hemagglutinin

 

 

QuikSep Sulfate Purification of rabies virus chromatography

 

1. The chromatography column

介质:QuikSep Sulfate 规格:1ml 预装柱

 

2. Sample pretreatment

高倍浓缩后的样品用平衡液稀释 20 倍,上样前 0.45um 过滤,0.45um 过滤。

 

3. buffer

A10mM PBpH 7.2 B10mM PB1M NaClpH 7.2

备注:所有溶液需 0.45um 过滤。

 

4. The purification process

流速:1ml/min 或者 30cm/h

平衡:溶液 A 平衡 10CVCV 指柱体积,下同);

上样:上样 30CV

清洗:溶液 A 平衡 10CV

洗脱:溶液 B 洗脱 10CV,收集洗脱液。

 

5. Purification of map

                       QQ截图20191022082740.jpg


5. Test results


体积

ml

蛋白浓度

ug/ml

抗原含量

IU/ml

蛋白去除率

%

收率

%

浓缩倍数

纯化倍数

原液

30

615

1.51

94

75.4

11

13

流穿液

30

557

0.22

洗脱液

2

531

17.08

 

7. conclusion

该悬浮培养狂犬病毒纯化工艺中,病毒与 QuikSep Sulfate进行吸附结合,洗脱液中蛋白去除率≈94%,回收率≈75.4% 

8. Order information

产品

规格(ml)

货号

QuikSep Sulfate

25

HZ1010-1

QuikSep Sulfate

100

HZ1010-1

QuikSep Sulfate

500

HZ1010-1

QuikSep Sulfate

1000

HZ1010-1


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